Product Overview

RAD-140 powder, commonly known as Testolone, is a brandless research-grade compound chemically designated as 2-chloro-4-[(1R,2S)-1-[5-(4-cyanophenyl)-1,3,4-oxadiazol-2-yl]-2-hydroxypropylamino]-3-methylbenzonitrile, provided exclusively for analytical laboratory and investigative research purposes. It serves qualified research institutions, pharmaceutical R&D laboratories, and analytical facilities needing a verified SARM analog for in vitro receptor binding studies, chromatographic method development, and structural characterization workflows. Each batch meets or exceeds ≥98% purity standards, supported by comprehensive analytical verification including certificates of analysis (COAs) for laboratory quality integration.

As an unbranded generic reagent, RAD-140 powder (Testolone) is positioned strictly for non-clinical research applications such as ligand affinity profiling, quantitative bioanalysis, and reference standard preparation. No implications for biological testing, therapeutic applications, or administration protocols are intended or supported. For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.

Chemical Identity & Classification

RAD-140 powder (Testolone) is classified as a nonsteroidal selective androgen receptor modulator (SARM) research chemical, serving as an analytical reference standard in pharmacology and receptor research contexts. Its specific stereochemistry at C1 and C2 positions, along with oxadiazole and benzonitrile pharmacophores, distinguishes it within SARM analog series for laboratory structural studies.

Key identifiers include:

• Synonyms: RAD140; Testolone; Vosilasarm

• CAS number: 1182367-47-0

• Molecular formula: C20H16ClN5O2

• Molecular weight: 393.83 g/mol

These specifications facilitate precise cataloging in laboratory information management systems (LIMS), mass spectral library matching, and standardized documentation for research protocols.

Chemical & Physical Characteristics

RAD-140 powder (Testolone) typically appears as a white to off-white crystalline solid, optimized for analytical stability and handling in powdered form. The fine crystalline morphology supports accurate gravimetric dispensing, dissolution into stock solutions (≥100 mg/mL in DMSO), and aliquoting for calibration standards or high-throughput screening formats.

Solubility demonstrates excellent compatibility with DMSO, with limited aqueous solubility (<0.1 mg/mL) characteristic of lipophilic SARM structures, requiring cosolvent strategies for buffered applications. XLogP3-AA value of 3.1 indicates favorable membrane permeability for cell-based assays, while topological polar surface area (119 Ų) informs ADME profiling in analytical contexts. Physical properties support room-temperature manipulations with desiccated storage to maintain crystalline form integrity.

Purity & Analytical Verification

Guaranteed ≥98% purity through orthogonal analytical confirmation including high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), nuclear magnetic resonance (NMR) spectroscopy, and chiral analysis confirming (1R,2S) stereochemistry. These methods quantify API content, resolve diastereomeric impurities, profile synthetic byproducts, and verify isotopic distribution against theoretical monoisotopic mass (394.1 [M+H]+).

Batch-specific COAs provide detailed chromatograms (>98% main peak area), mass spectral fragmentation matching reference libraries, 1H-NMR/13C-NMR assignments, and optical rotation data confirming enantiomeric excess. Documentation enables method transfer to CROs, instrument qualification per USP <1225>, and data integrity compliance for multi-site collaborations. Raw spectral files support custom reprocessing for publication-grade analytics. Third-party validation laboratories offer independent confirmation for proficiency testing requirements.

Quality Control & Batch Integrity

Batch manufacturing employs unique lot serialization linking primary packaging to immutable electronic records, ensuring complete supply chain traceability. Quality assurance encompasses raw material CoA review, in-process HPLC monitoring of coupling efficiency and oxadiazole cyclization, final release testing per ICH Q3A/B guidelines, and accelerated stability studies (40°C/75% RH) projecting 24+ month shelf life.​

Statistical process control (Cpk >1.67) governs critical quality attributes including polymorphic form (Form I anhydrous), particle size distribution (D90 <50 μm), and residual palladium (<10 ppm) from cross-coupling steps. Retained reference standards bridge production lots, essential for longitudinal receptor binding or pharmacokinetic studies. Internal quality independence and optional pharmacopeial IR matching enhance documentation suitability for NIH grant reporting and IND-enabling packages.

Safety, Handling & Laboratory Precautions

RAD-140 powder (Testolone) requires certified chemical fume hood manipulation by trained personnel to prevent dust inhalation, dermal absorption, or ocular exposure. Mandatory PPE includes 8-mil nitrile gloves, ANSI Z87.1 goggles, Tyvek labwear, and N95/P100 respirators for powder transfers >10 mg. HEPA-filtered local exhaust ventilation maintains airborne concentrations below internal action levels.​

Safety Data Sheet (SDS) review precedes use, detailing GHS classifications (H361f), incompatibilities with strong oxidants/acids, and spill response using vermiculite absorption followed by methanol extraction. RCRA hazardous waste protocols govern disposal; no OELs, acute toxicity data, or medical countermeasures provided. Institutional chemical hygiene plans dictate secondary containment and exposure monitoring.

Packaging, Labeling & Storage

Primary containment utilizes amber Type I glass vials with PTFE septa/crimp seals protecting against moisture ingress, photodegradation of cyano groups, and oxidative benzylic positions. Desiccant-packed secondary HDPE tubs with induction seals fortify transit integrity. GHS Rev.9 labels encode stereospecific IUPAC name, CAS registry, lot expiry, P260/P280 statements, and scannable DataMatrix COA access.​

Storage at -20°C under anhydrous nitrogen preserves chiral stability over 36 months; refrigerated (2-8°C) acceptable for <12 month horizons. Photoprotective wrapping prevents oxadiazole bleaching. HPLC stability-indicating assays (acid/base/oxidative/peroxide/light stress) inform requalification schedules. Segregate from Lewis acids, hypochlorites, and heavy metals precluding decomposition.

Intended Research Use & Market Positioning

RAD-140 powder (Testolone) supports androgen receptor pharmacophore optimization, tissue-selective SARM screening cascades, AR-positive breast cancer model interrogation, and quantitative LC-MS/MS bioanalysis method validation. Ki=7 nM potency enables fluorescence polarization binding displacement, TR-FRET coactivator recruitment, and BRET-based conformational assays in engineered cell lines.

Positioned exclusively as analytical reference material, excluding whole-organism PK/PD, human trials, veterinary applications, diagnostics, or therapeutic candidates. For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.

Ordering, Availability & Fulfillment

Immediate secure purchase via compliant eCommerce scaling milligram R&D through gram-scale optimization. PCI-DSS checkout integrates KYC verification, export control screening, and e-signature DEA declarations. Cold-chain fulfillment (2-8°C) employs datalogger-equipped carriers with RFID chain-of-custody.​

Custom services include autosampler aliquoting, 13C-D3 IS pairing, sterile filtration into LC vials, and scheduled replenishment keyed to study timelines. Account managers provide non-clinical solubilization protocols, counterion screening data, and orthogonal analytics. FIFO allocation delivers campaign-fresh material minimizing potency variance.

Legal & Regulatory Disclaimer

RAD-140 powder (Testolone) constitutes research chemical absent FDA/EMA approvals, GRAS status, NDI filings, or cGMP declarations. No specifications address pyrogenicity, bioburden, or ICH Q3D impurities. Purchasers affirm institutional authority, legitimate end-use, and compliance with DEA analogs, EU novel substances, TSCA inventory, and jurisdictional controls.

For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.