Product Overview

O-DSMT 50mg Pellets deliver a brandless, ≥98% purity research chemical in elevated dosage solid formulation precision-engineered for institutional analytical toxicology laboratories, forensic science facilities, and advanced pharmacological research departments requiring high-concentration certified reference standards for desmetramadol quantitative analysis, metabolic pathway reconstruction, and impurity profiling across UHPLC-MS/MS, GC-MS/NPD, and NMR analytical platforms. As tramadol’s principal pharmacologically active O-demethylated metabolite featuring (1R,2R)-trans-cyclohexyl configuration, this compound generates distinctive mass spectrometric daughter ions, gas chromatographic retention indices, and UV spectral signatures critical for validating screening cutoffs (typically 2-10 ng/mL), constructing comprehensive spectral libraries, and differentiating positional O/N-demethylation isomers in complex biological matrices or seized material investigations.

The standardized 50mg nominal active content per individual pellet provides exceptional batch homogeneity (RSD <1.0%) and quantitative content uniformity throughout extended production lots, enabling researchers to prepare stable working standards spanning 10-10,000 ng/mL concentration ranges with linearity coefficients r² ≥ 0.9999 across extended reversed-phase UHPLC gradients (C18, 100-250 mm × 2.1 mm ID, 1.7 μm) or LC-QqQ-MS/MS workflows monitoring primary MRM transitions 250→232, 250→58, 250→44 with optimized collision energies 15-35 eV. Higher dosage pellet specification facilitates preparation of bulk calibrator pools for external proficiency testing schemes, long-term stability evaluations, and high-throughput LC-MS method transfer between laboratories while maintaining sub-2% inter-assay precision across hundreds of injections.

Pellet matrix engineering incorporates directly compressible pharmaceutical-grade excipients (microcrystalline cellulose NF, anhydrous lactose Ph. Eur., crospovidone USP, colloidal silicon dioxide NF, magnesium stearate vegetable grade) ensuring >99.5% API dissolution within 15 minutes (USP Apparatus II paddle, 75 rpm, pH 4.5 acetate buffer simulating gastric extraction conditions) without gelation artifacts or dose-dumping phenomena characteristic of hydrophilic matrix systems. Manufacturing employs production-scale 16-station rotary compression (compression force 15-20 kN, turret speed 30 rpm) with in-process NIR spectroscopy confirming blend uniformity >99% prior to final compression, automated vision inspection rejecting defective units (>99.8% yield), and statistically designed 50-pellet content assay verification maintaining 50.0 ± 0.8 mg acceptance criteria. This material exists solely as an analytical reference standard for DEA-registered investigators operating within negative-pressure analytical laboratories possessing certified chemical fume hoods (100-150 fpm face velocity), high-efficiency particulate air filtration (HEPA H14, 99.995% efficiency), and validated controlled substance handling protocols compliant with 21 CFR 1301.13(e)(1) research quantity provisions. For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.

Chemical Identity & Classification

O-DSMT systematically identifies as (1R,2R)-3-(2-(dimethylaminomethyl)-1-hydroxycyclohexyl)phenol representing the pharmacologically predominant enantiomer of desmetramadol distinguished by trans-cyclohexane stereochemistry conferring 5-10-fold higher mu-opioid receptor affinity (Ki ≈ 10-30 nM) versus diastereomeric cis-configurations. Established analytical synonyms encompass O-desmethyltramadol, (+)-M1-tramadol metabolite, and desmetramadol positioning the compound within atypical synthetic opioid subclass structurally divergent from classical morphinan/phenylpiperidine pharmacophores while pharmacologically converging at mu-opioid receptor orthosteric site with additional norepinephrine transporter inhibition (IC50 ≈ 1-5 μM).

Molecular formula C15H23NO2 delivers exact mass 249.17288 Da verified through Orbitrap HRMS ([M+H]+ m/z 250.1807 ± 0.5 ppm), calculated formula weight 249.35 g/mol. Characteristic phenolic OH, tertiary amine, and tertiary alcohol moieties generate UV absorbance maxima 225/273/278 nm optimal for HPLC-DAD quantitation, while cyclohexyl backbone confers metabolic stability against rapid phase I oxidation relative to parent tramadol. Canonical SMILES notation CN(C)C[C@H]1CCCC@@(O)c2cccc(O)c2 and InChI=1S/C15H23NO2/c1-16(2)11-13-6-3-4-9-15(13,19)12-7-5-8-14(18)10-12/h5,7-8,10,13,18-19H,3-4,6,9,11H2,1-2H3/t13-,15+/m1/s1 enables automated spectral library matching essential for high-throughput unknown screening platforms.

CAS 80456-81-1 (racemic specification) with (1R,2R)-(+)-enantiomer tracked through ChEMBL CHEMBL1200658, PubChem CID 9838803, and international forensic substance databases. Universal classification designates O-DSMT as Schedule I-equivalent analytical reference within opioid metabolism research and novel psychoactive substance monitoring frameworks absent clinical development pathways.

Chemical & Physical Characteristics

50mg pellet dosage units manifest as uniform 6-7 mm diameter × 3.5 mm biconvex off-white tablets exhibiting controlled surface roughness (Ra < 1.5 μm) and batch-specific visual coding through pharmacologically inert iron oxide pigmentation maintaining baseline transparency across 200-400 nm diode array detection windows. Direct compression formulation ensures quantitative API extraction (>99% recovery) via 10-minute vortex methanol dissolution or direct LC injection following aqueous dilution without matrix-derived ion suppression during ESI+ or APCI+ ionization.

Thermodynamic profile reveals glass transition Tg ≈ 45°C for amorphous excipient blend supporting ambient storage stability, with thermal decomposition onset >170°C permitting electron impact GC analysis (30 m × 0.25 mm DB-5MS, 1 μL splitless injection, 70-300°C @ 15°C/min) generating signature ions m/z 250 [M+H]+, 232 [M-H2O]+, 174 [cyclohexyl fragment]+, 58 [C3H8N]+ base peak distinguishing O-DSMT from N-demethyltramadol positional isomer. pKa values 9.4 (phenolic), 9.8 (aliphatic amine) support isocratic C18 retention (k’ = 4.2-5.8) across pH 3-7 mobile phases with logP 2.15 partitioning behavior.

Purity & Analytical Verification

Production release requires ≥98.5% purity confirmation through orthogonal methodology: UHPLC-DAD multiwavelength (225/273/278 nm, 100 × 2.1 mm Kinetex EVO C18 1.7 μm), LC-QqQ-MS/MS (ESI+ MRM 250→232/58/44 CE 12-38 eV), quantitative 1H-NMR (500 MHz D2O maleic acid IS), chiral HPLC (Chiralcel OD-RH, ee ≥ 99.5%), KF titration (<0.1% residual H2O). Impurity limits enforce tramadol <0.1%, cyclohexanone <0.05%, N/O-bis-demethyl metabolites <0.08% via peak area normalization.

Quality Control & Batch Integrity

Enterprise LIMS integration spans incoming CoA-qualified API through automated 60-pellet content uniformity assay (50.0 ± 0.6 mg), ICH Q1A 36-month stability cohorts confirming 99.2% assay retention under 25°C/60%RH accelerated conditions. ISO 17025 third-party orthogonal LC-HRMS verification (250.1807 ± 0.0003 Da).

Safety, Handling & Laboratory Precautions

Class II biosafety cabinet operations mandate 20 mil nitrile gloves, Z87+ chemical splash goggles, Tyvek® Level B ensembles per institutional controlled substance SOPs. GHS Category 2 irritant SDS details vinegar neutralization, RCRA D001 incineration protocols.

Packaging, Labeling & Storage

Induction-sealed HDPE bottles (silica gel Type B desiccant) within opaque secondary fiber drums. GHS labels specify UN3334 P5 exempt quantities, NFPA 704 H2-F1-R0, 36-month expiry. Controlled room temperature storage 15-30°C.

Intended Research Use & Market Positioning

Mu-opioid receptor characterization (Ki 10-30 nM), LC-MS toxicology screening (LLOQ 0.1 ng/mL whole blood), seized material quantitation, CYP2D6 metabolic phenotyping standards.

Ordering, Availability & Fulfillment

Secure institutional eCommerce platforms, ambient validated logistics, 24/7 analytical method support.

Legal & Regulatory Disclaimer

Exclusively analytical research chemical prohibiting human/veterinary/clinical/consumptive deployment. Purchaser verifies jurisdictional compliance. For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.