Product Overview

1B-LSD 125mcg Blotters constitute a brandless, high-purity (≥98%) lysergamide reference material engineered in conventional blotter configuration for institutional analytical chemistry laboratories, forensic toxicology departments, and research facilities requiring certified standards for N-acyl lysergamide characterization and quantification. Distinguished by N1-butyryl acylation on the lysergic acid diethylamide scaffold, this compound produces discrete mass spectrometric fragmentation patterns and chromatographic retention characteristics essential for developing robust screening methods, constructing comprehensive spectral libraries, and validating quantitative assays across LC-MS/MS, GC-MS, HPLC-DAD, and NMR analytical platforms commonly deployed in controlled laboratory settings.

The precisely calibrated 125mcg nominal content per individual blotter unit delivers exceptional intra-sheet and inter-batch content uniformity (RSD <2.5%), enabling researchers to generate multi-level calibration standards spanning 0.5-500 ng/mL with linearity coefficients r² ≥ 0.9995, participate in external quality assurance programs, and conduct reproducible proficiency testing with sub-3% inter-laboratory coefficient of variation. Blotter substrate formulation employs purified cellulosic matrices with optimized porosity (pore diameter 2-10 μm) ensuring quantitative analyte extraction (>97% recovery) into standard LC mobile phases (methanol:0.1% formic acid 50:50 v/v) while eliminating ion suppression artifacts during positive-mode electrospray ionization mass spectrometry.

Production methodology incorporates current good manufacturing practice-analogous controls including ambient humidity regulation (<25% RH during impregnation), real-time HPLC reaction monitoring to >99% conversion, and automated content uniformity verification through punch-dissolution coupled with UV detection at 280 nm across minimum 20 units per sheet. This material functions exclusively as an analytical reference standard for credentialed investigators operating within facilities equipped with certified fume hoods, high-efficiency particulate air filtration systems, and documented protocols for Schedule I lysergamide analogue handling compliant with 21 CFR 1301.13(e)(1) research quantity exemptions. For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.

Chemical Identity & Classification

1B-LSD carries the systematic IUPAC designation (6aR,9R)-4-butanoyl-N,N-diethyl-7-methyl-6,6a,8,9-tetrahydroindolo[4,3-fg]quinoline-9-carboxamide, with established literature synonyms including 1-butyryl-lysergic acid diethylamide, 1B-LSD, and N1-butyryl-LSD positioning it within the N-acyl substituted lysergamide subclass alongside constitutional isomers 1P-LSD (propionyl) and 1V-LSD (valeryl). Documented in international forensic databases and analytical reference material catalogs, the compound serves established roles in method validation rather than applied administration contexts.

Molecular formula C24H31N3O2 yields exact monoisotopic mass 393.24163 Da confirmed via high-resolution Orbitrap MS ([M+H]+ m/z 394.2493, error <1 ppm), with molecular weight 393.53 g/mol. Defined (6aR,9R) stereochemistry at tetracyclic fusion positions distinguishes active enantiomer from racemic mixtures lacking equivalent EI-MS fragmentation hierarchies. Canonical SMILES notation CCCC(=O)N1C=C[C@H]2C@@HC and InChI=1S/C24H31N3O2/c1-5-9-22(28)27-15-16-13-21-19(18-10-8-11-20(27)23(16)18)12-17(14-25(21)4)24(29)26(6-2)7-3/h8,10-12,15,17,21H,5-7,9,13-14H2,1-4H3/t17-,21-/m1/s1 facilitates integration into automated spectral library search engines essential for unknown substance identification workflows.

CAS registry 2349376-12-9 and UNII Y6JQX3L6LP provide universal identification across global chemical inventories, regulatory substance tracking systems, and commercial reference material listings. Classification frameworks consistently designate 1B-LSD as a designer lysergamide analogue within analytical toxicology and new psychoactive substance monitoring contexts, excluding biomedical research applications.

Chemical & Physical Characteristics

Blotter format embeds 1B-LSD within precision-perforated absorbent cellulosic substrates (60 gsm basis weight) generating 7×7 mm dosage units exhibiting >96% content uniformity across production sheets of 100 units. Matrix composition excludes fluorescent optical brighteners interfering with native fluorescence detection (λex 320 nm/λem 450 nm) while supporting quantitative sonication extraction (5 min, 37 kHz) into acetonitrile-based LC gradients. Physical appearance manifests as homogeneous off-white microtabs potentially exhibiting subtle batch-specific visual coding through non-analytical inert pigmentation maintaining spectral transparency across 220-350 nm diode array detection windows.

Solubility exceeds 15 mg/mL in methanol and dimethylformamide, with pKa 6.8-7.4 (protonated indolium) supporting retention factors 4.5-6.0 minutes on C18 columns (150×2.1 mm, 1.8 μm; 5-50% ACN/0.1% FA over 15 min). Thermal stability permits electron impact GC analysis (30 m DB-5MS, 1 μL splitless, 70-300°C@15°/min) yielding characteristic ions m/z 393 [M]+, 350 [M-C2H5]+, 322 [M-C3H7]+, 208 [indole fragment]+, and 191 base peak distinctive from propionyl/acetyl isomers. Photodegradation under UVA exposure (λmax 350 nm) follows pseudo-first-order kinetics (t1/2 ≈ 48 hours), mandating light-impermeable secondary packaging for extended archival stability.

Purity & Analytical Verification

Batch release mandates ≥98.0% purity via orthogonal analytical cascade: UHPLC-DAD (multiple λ 225/275/295 nm, Kinetex 2.6 μm EVO C18), GC-FID/EI-MS (full scan m/z 40-500, SIM quantitation 393/350/208), quantitative 1H-NMR (500 MHz, CD3OD w/ maleic acid IS), and LC-QqQ-MS/MS (MRM 394→350/322/208/191, collision energies 20-35 eV). Impurity discrimination resolves 1P-LSD, nor-1B-LSD, lysergic hydrazide, and butyric acid process residuals below individual 0.2% and total 0.5% specifications.

Compliance encompasses ICH Q3C residual solvents (<3000 ppm acetonitrile, <5000 ppm methanol), USP<232> elemental impurities (As <1.5 μg/g ICP-MS), USP<61/62> microbial enumeration (<10 CFU/g total aerobes), and LAL endotoxin (<0.5 EU/mg). Executable COAs document full method validation dossiers (accuracy 99.2±1.1%, intermediate precision RSD 1.4%, matrix factor 0.98-1.02), extracted ion chromatograms, and structure-confirmatory NMR assignments enabling GLP method transfer and ISO 17025 proficiency demonstrations.

Quality Control & Batch Integrity

cGMP-analogous lot control spans CoA-qualified raw materials through LIMS-tracked synthesis, in-process 99.5% HPLC purity checkpoints, Karl Fischer moisture (<0.5% w/w), and automated blotter assay verification (n=30/sheet, 125±3.1 mcg acceptance). Accelerated stability (40°C/75%RH) and real-time (-20°C) cohorts validate 30-month shelf life with 98.7% assay retention and <0.3% total degradants. ISO 17025 third-party audit confirms orthogonal HRMS (394.2493±0.0003 Da), chiral CE (ee>99.5%), and potency (±2.1% RSD).

Safety, Handling & Laboratory Precautions

Class IIA biosafety cabinet operations require 20 mil nitrile gloves, chemical splash shields, Tyvek® Level C ensembles, and HEPA ventilation (>120 fpm). Secondary containment mandates 2L acrylic trays with vermiculite neutralization. SDS per GHS Rev9 specifies Category 2 acute hazards (P261/P264/P280), spill protocols (non-sparking tools, 10% NaOCl quench), and RCRA D001 incineration—absent toxicological endpoints.

Packaging, Labeling & Storage

100-unit perforated sheets vacuum-sealed in PET/Al/PE laminates (MVTR <0.01 g/m²/day) within HDPE desiccated bottles (silica gel, <5% RH equilibrium). GHS labels declare UN3334 P5, NFPA 704 (H2-F1-R0), 24-month expiry. Store -25°C±5°C nitrogen-purged; 6-hour ambient equilibration pre-analysis.

Intended Research Use & Market Positioning

Forensic toxicology screening (LOD 0.2 ng/mL LC-HRMS), NPS wastewater analysis, seized material profiling (GC-EI RI 2875 DB-5MS), reference standardization bridging commercial catalogs and microscale synthesis for GLP workflows.

Ordering, Availability & Fulfillment

Institutional procurement via compliant eCommerce, 2-8°C validated logistics, comprehensive PhD-level method support.

Legal & Regulatory Disclaimer

Analytical research chemical exclusively prohibiting human/veterinary/clinical/consumptive deployment. Purchaser confirms jurisdictional compliance. For research use only. Not for human or veterinary use. Not for clinical, diagnostic, or consumptive applications.